![]() Even though lentivirus is the customary method, there is still hesitation because an extensive safety profile is still being established and understood. That is why there is an immense interest in a delivery technology that will increase flexibility and improve delivery efficiencies. The Neon Electroporation system is an easily adaptable solution in which successful delivery of mRNA in primary T-cells has been shown to achieve greater than 75% efficiency. With minimal optimization, we will show how the Neon system can be used for immune cell-based applications. ![]() XĪttune NxT Flow Cytometer with Automated Acquisition Sampler as a High Throughput Screening Tool – Multiplexed Flow Cytometry Used to Analyze Metabolic Activity and Assess Pharmaceutical Compound Toxicity These technologies can be applied to help advance research within immunology, immunotherapy, and immuno-oncology. Quentin Low, Staff Scientist, Life Sciences Group, Thermo Fisher Scientificįlow cytometry has recently emerged as a powerful HTS tool adding the benefits of single cell multiparametric analysis.We compared multiplex flow cytometer and single-read plate reader HTS platforms by screening drug compound libraries on Jurkat and Ramos cell lines. #Seattle medical conferences 2016 seriesĬells were cultured under either normoxic (5%) or hyperoxic (19%) conditions grown in the presence of the Microdiscovery Discovery “Killer Plate” drug compound library under a series of time points (24, 48, or 72 hrs). The two oxygen levels simulate cellular oxygen conditions in either a tumor environment or normal cellular environment. #Seattle medical conferences 2016 series. ![]()
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